【病毒外文文獻(xiàn)】2017 Development of Different Animal Models for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) Infection

ENDOMETRIAL LESIONS UNDERLYING FELINE PYOMETRA M A Pires H Vilhena y S Miranda y M Tavares Pereira z F Seixas A L Saraiva and R Payan Carreira CECAV LaboratC19orio de Histologia e Anatomia PatolC19ogica UTAD Vila Real y Baixo Vouga Veterinary Hospital C19 Agueda and z Vet4 Estarreja Portugal Introduction Pyometra is an important uterine disease in cats and usually secondary to other uterine lesions often associated with cystic endometrial disease cystic endometrial hyperplasia pyometra com plex CEH Pyo However recent evidence points to the fact that pyometra also accompanies feline endometrial adenocarcinoma FEA Failure to properly analyze through histopathology any pyo metra case may permit the spread of a more aggressive undetected neoplasia This work aimed to draw attention to the need for careful histopathological examination of the excised feline genital tract particularly when areas of increased mural thickness are noted Materials and Methods We evaluated the records of 271 feline ovariohysterectomy OVH specimens from laboratory archives within the last 7 years Results In 271 OVH specimens 125 had lesions CEH was the most frequent finding 44 followed by pyometra 30 4 and FEA 25 4 Concomitant with pyometra we found FEA in 47 3 of cases Only in 13 2 of cases pyometra was CEH observed simultaneously Conclusions All OVH specimens should be carefully examined as lesions of FEA could be masked FEA is usually described as a rare pathology in cats but recent reports suggest that it may be more frequent than thought Age is not an adequate factor for triage since some FEA cases were described in young animals and pathologists and clinicians should be advised of this 66 ESVP and ECVP Proceedings 2016 J Comp Path 2017 Vol 156 54e141 17 BETA OESTRADIOL REGULATES OXYTOCIN PRE PROPEPTIDE mRNA EXPRESSION IN CULTURED BOVINE SATELLITE CELL DERIVED MYOTUBES E Berio S Divari P Pregel E Bollo B Biolatti and F T Cannizzo Department of Veterinary Sciences University of Turin Italy Introduction Oestrogens induce oxytocin OT expression in several tissues by classical and non classical signalling The aim of this study was to evaluate the role of oestrogen receptor a ORa in OT mRNA expression in cultured bovine satellite cell BSC derived myoblasts Materials and Methods BSCs were isolated from the deep dig ital flexor muscle of adult male cattle At day 8 differentiation was induced and myotube development was demonstrated by spe cific immunofluorescence labelling Myotubes at day 14 were treated with 17 beta oestradiol 17B O with and without ORa antagonist ICI 182 780 for 24h and mRNA levels of OT pre pro peptide and insulin like growth factor 1 IGF1 were measured by qPCR Results OT mRNA level in myotubes was significantly upregulated and this effect was not suppressed by ICI IGF1 mRNA was mildly upregulated by 17B O treatment and significantly upregulated in 17B O ICI treated myotubes Conclusions 17B O induces OT mRNA expression in BSC derived myotubes as previously described in bovine skeletal muscle in vivo ICI exerts its antagonist activity by inhibition of the binding of the ORa ligandcomplextoDNA andithasbeendescribedasanactivator of the orphan G protein coupled receptor 30 GPR30 The IGF1 in crease induced by 17B O with ICI treatment is suggestive of GPR30 involvement ICI does not influence OT overexpression induced by 17B O suggestingthat17B Omayexertits effecton OTtranscription through nuclear orphan receptors This is the first description of in vi tro OT mRNA upregulation induced by 17B O in BSC derived myo tubes suggesting a role for OT in skeletal muscle differentiation DEVELOPMENT OF DIFFERENT ANIMAL MODELS FOR MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS MERS COV INFECTION J Vergara Alert J Van den Brand y W Widagdo y M Mu noz D Solanes A Bensaid B Haagmans y and J SegalC19es z Centre de Recerca en Sanitat Animal CReSAeIRTA Spain y Department of Viroscience Erasmus Medical Centre The Netherlands and z Departament de Sanitat i Anatomia Animals Universitat AutC18onoma de Barcelona Spain Introduction Middle East respiratory syndrome coronavirus MERS CoV emerged in 2012 as a causative agent of severe pneu monia and mortality in man Further research indicated that an an imal reservoir for MERS CoV did exist dromedary camels Since other animal species susceptible to this viral infection cannot be ruled out the aim of this work was to test four different species for their susceptibility to MERS CoV and potential as an animal model Materials and Methods A total of 14 pigs 14 sheep eight horses ponies and eight llamas were inoculated intranasally with 10 7 TCID 50 animal Four pigs and four sheep were killed on day 2 post inoculation pi and four animals of each species were killed on days 4and24pi Acompletenecropsyexaminationwasperformedonallan imals and histopathology and immunohistochemistry was performed onasetoftissues withemphasisontherespiratorytract Virusisolation and RT PCR to detect MERS CoV was performed on nasal swabs collected at 1 2 3 5 7 10 15 and 26 days pi Results Only llamas showed clinical signs consisting of moderate nostril mucus secretion Inflammation and necrosis was predomi nantly seen in the nasal respiratory epithelium of llamas and pigs Only pigs and llamas had positive virus isolation and RT PCR results in nasal swabs variably from days 1 to 10 Conclusions Pigs and llamas are susceptible to infection with MERS CoV and might be used as potential models of subclinical infection with this virus